FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761491
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG134260.3569329289901265No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG96940.2577169531975485No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC95600.25415453606030164No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC63930.16995919968969753No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA54210.14411838284339906No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT53520.14228400386974208No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT49500.13159675245800137No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG42760.11367832596169976No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG41270.10971713078670134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA38700.037.12423732
GCGGCAC41150.035.00478733
TCGCCGG38750.034.78022848
CGGCACC42000.034.14063334
CCGTCGC40100.034.01261545
CCCGTCG40900.033.68041244
TGGCCGT14200.032.93362470
ACCCGCG44750.032.07186525
CCCCCGT43400.031.64703642
CCCCCGA45750.031.50100119
CCCCGAC47300.030.39791320
GCACCCC49100.029.78021236
CCGCGCG49050.029.33953727
CACCCCC50150.028.74998537
TAGTCCG3950.028.0536986
GGCACCC51600.027.99484835
AGTCCGC52000.027.5698417
GCGCGGG50250.027.45589362
GATAGGC28750.026.96352640
CGTCGCC50950.026.70834746