FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761491
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113980.3030181382861211No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA113400.30147619654014857No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC104430.2776292698826077No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG79590.21159162683095614No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT48090.12784823890313707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG19250.060.4235764
TCTACGG20300.057.2982185
TACGGCC20450.057.044947
CTACGGC21700.053.4420286
GCGCGGT59100.052.070879
CGCGGTG63800.048.45195810
TGGCGCG63200.048.36357515
ACGGCCA24200.048.3483778
GCCATAC24500.047.4737711
CGGCCAT24950.047.1725049
GAACGCG24400.046.44215424
ATCTCGT24400.046.08910435
GCCGGTT8850.044.9834183
TGAACGC25400.044.8818123
CCCGATC25450.044.42539631
CCATACC26700.043.82079312
CCGAACT8700.043.76056367
CTGAACG26300.043.47131322
GCCCGAT25900.043.37704530
TCTCGTC26150.042.74669336
Produced by FastQC (version 0.11.5)