FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766509
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113090.30025150610286605No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA110910.29446365321309464No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC100610.2671173757981197No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80730.21433640540882817No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47620.12643007092243772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG18900.059.1737635
GTCTACG19250.058.4576154
TACGGCC19250.058.4560627
CTACGGC20850.053.9687966
GCGCGGT59900.052.7741059
TGGCGCG63250.049.7039315
ACGGCCA23050.048.969278
CGCGGTG66250.048.02933510
CCGAACT9400.046.5137367
GCCATAC24100.046.40474711
CGGCCAT24700.045.838229
CCATACC24850.045.1435312
CGAACTT10150.044.98536768
GAACGCG25100.044.72300324
GGGTCTA20950.044.4598772
CGGTTCA10300.044.373655
ATCTCGT25200.044.3412735
CTGAACG25350.043.99807722
CGTGCCT71800.043.65265720
TGAACGC25800.043.5061223
Produced by FastQC (version 0.11.5)