FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758159
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG136010.36190592255410164No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG97790.2602071918724035No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC95030.25286317050449436No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC66470.17686851461047814No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA55330.1472263414081203No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT54100.14395346232024775No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT50720.1349596970218663No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG44280.11782364716341166No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG40980.10904275204960726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA40150.036.21805232
GCGGCAC42850.034.1834733
CGGCACC42950.033.7842334
TCGCCGG41050.033.08251648
CCGTCGC42750.032.06230545
CCCGTCG43350.031.93185244
CCCCCGA46900.031.10530719
TGGCCGT12600.030.82919770
ACCCGCG47700.030.45699125
CCCCGAC48250.030.23822620
GCACCCC50300.028.92815836
ACGATCG605.2972714E-428.8668546
CCGCGCG50650.028.82768427
CCCCCGT48350.028.32870942
AGTCCGC53800.027.622047
CAGTCCG51150.027.4277446
CACCCCC53100.027.4086137
ATGGCCG11400.026.89177769
GATAGGC29050.026.43585440
CCCGCGC55000.026.35463726