FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542214
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG187200.5284830334926123No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC111990.31615819936344897No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG81790.23090078690897842No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC81110.22898108358218897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80390.22694845653029433No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG64900.18321874398328278No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49900.14087234706881063No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG48340.13646832178970555No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA45680.1289588940702058No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT45360.12805550426936374No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40840.11529512333246945No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37430.10566837576724614No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC35650.10064327000006211No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG35430.10002218951198319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA59550.037.9905332
GCGGCAC62450.036.2439133
TCGCCGG61200.035.531248
CGGCACC64350.035.19282534
CCCGTCG62400.034.70509744
CCGTCGC62800.034.3386245
TGGCCGT14050.032.72918370
CCCCCGA69450.032.6412919
ACCCGCG69850.032.57150325
CCCCGAC71500.031.764420
GCACCCC64550.031.40706636
CCCCCGT70250.030.59599542
GGCACCC66300.030.5135235
CCGCGCG74850.030.46735427
GTTCCGT4100.029.4987641
GCGCGGG77450.028.4668862
CCCGCGC80650.028.21926
TCAGGAT20250.027.94879370
CACCCCC73500.027.69491637
CGTCGCC78800.027.473446