FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542214
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC148090.41807186127094526No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC125100.3531689502666976No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA116070.32767641932418534No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG99660.2813494610997529No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80020.22590391207307067No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63320.17875825684162505No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC44920.1268133432932059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC22100.060.112987
TCTACGG22300.059.8808255
GTCTACG22250.059.706034
CTACGGC23200.057.7062076
GCGCGGT71400.053.216229
CCGAACT8300.051.89153767
ACGGCCA25750.051.8594258
CGGCCAT26300.050.3823249
TGGCGCG75750.050.15385415
GCCATAC26250.049.94606411
GGGTCTA21150.048.1614152
CGCGGTG79350.048.05803710
CATACCA28350.046.007513
GCCGGTT9500.045.2797783
CCATACC29450.044.2890512
CGGTTCA9650.044.223725
CGTGCCT86350.044.04453320
GGCCATA30200.043.87599610
GAACGCG30000.043.69756324
CGGCAAT2950.043.370311