FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520088
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG183690.5218335450704641No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC112240.31885566497201207No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG83550.23735202074493594No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC79290.22525005056691763No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76950.21860248948321745No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63260.17971141630550147No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT48940.13903061514371232No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46070.13087740988293475No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA44350.1259911684026081No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT43690.12411621527643626No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40010.11366193117899326No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA38640.10976998302315169No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC35590.1011054269097818No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG35270.10019635872739545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA58550.037.86126332
GCGGCAC60000.036.9648133
TCGCCGG58650.036.1564348
CGGCACC61800.035.8408234
CCGTCGC59950.035.4821245
CCCGTCG60750.035.10385544
CCCCCGA67950.033.02262519
ACCCGCG67950.032.68202625
GCACCCC62550.031.946436
CCCCGAC70500.031.64209720
TGGCCGT13650.031.23444770
CCCCCGT68150.031.20818542
GGCACCC64600.030.49119235
CCGCGCG73100.030.45287327
GTTCCGT3250.029.923081
TACGGGT12050.029.430676
GATAGGC33250.029.28260640
TAGTCCG4050.028.905066
GCGCGGG75800.028.38669262
CGTACTA854.4996086E-628.3561742
Produced by FastQC (version 0.11.5)