FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520088
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC147310.41848385608541605No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC125220.35572974312005834No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA115010.3267247864257939No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG100250.28479401651322356No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76570.21752297101663368No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61730.1753649340584667No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC44420.12619002706750512No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG35270.10019635872739545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC21400.059.6781437
TCTACGG21600.058.806835
GTCTACG22000.057.5811464
CTACGGC23050.055.2568326
GCGCGGT69600.054.4026579
CCGAACT10550.051.98099567
CGGCCAT25000.051.0823149
ACGGCCA25300.050.7487038
TGGCGCG75100.050.41915515
GCCATAC25700.049.69096411
CGCGGTG77900.049.0039110
CGGTTCA11500.048.192925
GCCGGTT11700.047.074893
CCATACC27350.046.69315312
CATACCA27300.046.52715713
GGGTCTA20000.046.482332
CCGGTTC11800.046.3842244
GGCCATA28150.045.73301710
CGAACTT12750.044.63517868
TACCACC28750.044.42003615