FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520613
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG187100.5314415415724477No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC111890.31781397160096836No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC82400.23405014978925545No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG81400.23120973535006545No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77010.21874031596202137No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62230.1767589905507933No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT50560.14361135404544606No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG48670.13824297075537698No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA44840.1273641834532793No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT42470.120632401232399No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40550.11517880550915424No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA38800.11020808024057174No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG36620.10401597676313755No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC35880.10191407007813695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA62400.038.31176432
TGGCCGT14300.038.16131270
GCGGCAC64650.036.8339933
CGGCACC66400.036.03428334
TCGCCGG63850.035.02763448
CCCGTCG65050.034.67834544
CCGTCGC64950.034.63735645
TAGTCCG3150.033.8749356
ACCCGCG71700.033.36886625
CCCCCGA72250.033.2910419
CCCCGAC73750.032.5774220
GCACCCC66050.032.37455436
GTTCCGT3250.031.8892521
CCGCGCG75950.031.39302827
GGCACCC69000.030.82003435
CCCCCGT73550.030.54017642
CACCCCC73400.029.05061337
CCCGCGC84000.028.77986326
ATGGCCG15350.028.77985869
GCGCGGG79700.028.56853162