FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502535
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC143490.4096747070336199No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC119290.340581892829051No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA107820.30783418295605897No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG97540.2784840122939528No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78210.22329541317931156No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT62840.17941291093450884No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42290.12074112035996785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG20000.060.0832185
GTCTACG20350.059.3737874
TACGGCC20150.059.2933437
CTACGGC21300.056.0928656
GCGCGGT68050.052.671319
CCGAACT9900.051.7699467
TGGCGCG71400.050.1410415
ACGGCCA24000.049.9251678
GCCATAC23950.049.8856311
CGGCCAT24100.049.5751389
CGGTTCA10550.048.6285745
CGGCATA2600.048.0439221
CGCGGTG75550.047.71596510
GCCGGTT11000.047.567373
GGGTCTA20400.046.4152032
CCATACC26100.046.1641112
CATACCA25800.045.90031413
GGCCATA27000.044.5054510
CGTGCCT82700.043.38612420
GTAGTCC84150.043.15077227