FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4980290
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG198070.3977077640057105No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC149030.29923960251310666No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC127400.2558083966997906No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA77030.1546697079888922No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT72620.1458148019492841No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG59570.1196115085667702No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC49870.10013473111003576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG44650.062.431094
TCTACGG44550.062.338625
TACGGCC44500.061.864067
CTACGGC46300.060.131626
ACGGCCA49100.055.9978948
CGGCCAT49700.055.254039
GCCATAC50850.054.00768711
GCGCGGT81350.052.567379
GGGTCTA38050.051.7554782
CCATACC54200.050.7975512
GCGTCTA10950.050.482632
CATACCA55500.049.8566713
CCTGGTT79150.049.17748670
GGCCATA56100.049.01260410
TGGCGCG89600.048.11609315
CTGAACG57700.048.0595422
GGTCTAC45950.047.817393
CGCGGTG90150.047.474810
GCCGGTT12600.047.434013
CGTCTAC13150.047.2890973