FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5023693
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG206810.4116692640254888No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG89410.17797663989419735No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC61880.12317631670565857No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG58720.11688612341558292No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54110.10770960725506117No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG52180.10386781198612256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT23700.039.23711870
CGCGGCA61350.035.6412232
GCGGCAC64050.034.14491733
CCCGTCG63500.033.29835544
TCGCCGG63500.033.2544648
GTTCCGT6150.033.188111
CCGTCGC63600.032.9894545
CGGCACC66900.032.90608634
ATGGCCG24350.030.62079269
GCACCCC75600.029.40939936
GGACGTA5600.029.17678343
CCCCCGA77450.028.57104719
CACCCCC78000.028.33483337
GACGTAT5700.028.06569944
CGTACGT1850.028.03413419
ACCCGCG79950.027.69888925
GGCACCC81650.027.0946935
AGTCCGC85750.026.1999057
CCCCGAC85550.025.90915320
CAGTCCG83250.025.6993796
Produced by FastQC (version 0.11.5)