FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5023693
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG206060.41017633840284434No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC160210.3189088186718416No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC136340.27139397252180814No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA82110.16344549716712387No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT75450.1501883176380404No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG60680.12078763570942731No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC53530.10655507810688272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG44100.062.8439254
TACGGCC44650.061.837637
TCTACGG44900.061.6472555
CTACGGC45850.060.4453056
ACGGCCA48900.056.745878
GCCATAC51250.054.0068611
CGGCCAT51500.053.612639
GCGCGGT85100.051.6111539
CCATACC53950.051.3040112
GGGTCTA39200.051.140462
CATACCA54250.051.08413
GCGTCTA10850.050.332682
TCGCGTA352.043287E-550.18531460
CGTCTAC12800.049.4047473
CCTGGTT79650.048.79832570
GGCCATA57400.048.1602110
CTGAACG58150.047.63310222
TACCACC58250.047.4583815
GAACGCG58600.047.2210224
TGGCGCG93350.046.71581615
Produced by FastQC (version 0.11.5)