FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5161145
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG212140.4110328231429266No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG91840.17794501026419526No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC61680.11950836490739943No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG59670.11561388025331587No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG56780.11001434759147437No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG51720.10021032154686606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT23800.038.84620770
CGCGGCA65450.035.4741232
GCGGCAC69250.033.58769233
TCGCCGG67850.032.97489548
CGGCACC71500.032.58555634
CCCGTCG69200.032.17017444
CCGTCGC69100.032.02599745
GACGTAT4850.030.83941844
ACCCGCG79500.029.46923625
CCCCCGA80750.029.15503719
GGACGTA5400.028.97450443
ATGGCCG25250.028.91517369
GCACCCC81850.028.65124536
CACCCCC82900.028.21234737
GTTCCGT6450.027.9010471
CGTATCA5500.027.21840146
CCCCGAC87550.026.85368720
ACGTATC5600.026.71800445
GGCACCC88550.026.5545135
CGGATAG5750.026.53884135