FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5092432
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG205060.4026759709309815No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG89960.17665429798571683No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC61700.12116018436770487No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG58450.11477816493180469No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54870.10774812506087465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT24050.045.10579370
CGCGGCA63200.037.11721832
ATGGCCG23150.036.78907469
GCGGCAC65950.035.7888233
CGGCACC69450.033.69208534
TCGCCGG65600.033.66404748
CCGTCGC66100.033.41578345
CCCGTCG66850.033.2371944
CCCCCGA78150.030.61637919
ACCCGCG78650.030.00777625
GCACCCC78900.029.84599136
GTTCCGT7050.029.4708291
CGTACGT2700.029.45376419
CACCCCC80500.029.3476237
AGGACCG9750.029.25170770
CCCCGAC85600.027.7934620
CGGATAG5750.027.73861535
AGTCCGC86100.027.5029187
GGACGTA5600.027.31164743
GGCACCC85900.027.28653135