FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5092432
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG203760.40012316315662144No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC157820.3099108638073125No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC136250.267553891735815No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA83280.16353679342208202No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT75760.148769782296553No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG61590.12094417755602824No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC51420.10097336596738062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG45400.060.743214
TCTACGG45300.060.5757265
TACGGCC45250.060.3371477
CTACGGC46900.058.4354826
ACGGCCA49300.055.2402428
GCGCGGT86550.053.2678349
CGGCCAT51450.052.9318589
GCCATAC51750.052.49144411
CCATACC53950.050.3504212
CATACCA55000.049.64053313
GGGTCTA40900.049.5136572
TGGCGCG93600.048.8864415
CCTGGTT79800.047.9919370
CGCGGTG96150.047.80558810
GGCCATA57150.047.4711510
CCGAACT13200.047.33526267
GCGTCTA10900.047.2400632
CTGAACG57950.047.20471222
TACCACC59100.046.08027315
CGTCTAC12700.045.986853
Produced by FastQC (version 0.11.5)