FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896402
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG102320.3532658795291538No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71800.24789376612776814No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61030.21070970120860297No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44500.15363889404854714No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38220.1319568209109095No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36340.12546600920728546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21100.061.501774
TACGGCC20950.061.283857
TCTACGG21250.061.0676465
CTACGGC22100.058.718896
ACGGCCA24550.052.577638
CGGCCAT24600.052.0519039
GCGCGGT38350.050.622379
GCCATAC25000.050.5315111
CCATACC25900.049.04224812
TGGCGCG40350.048.7973515
CATACCA26050.048.75985713
GGGTCTA20900.048.2650452
CCGAACT6950.046.85716667
GCGTCTA4950.046.5994722
CCTGGTT40050.046.11810370
GAACGCG27700.046.07925824
CGCGGTG42450.045.7338410
GGCCATA28150.045.2438510
TGAACGC28350.045.133523
CTGAACG28600.044.84946422
Produced by FastQC (version 0.11.5)