FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885752
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG107300.3718268236494335No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57830.2003983710311905No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47800.16564139953814466No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA33510.11612224473897964No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC32780.11359257482971509No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC28860.10000859394708901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT11850.038.64208670
CGCGGCA35950.037.06317532
GCGGCAC37750.035.0349533
CGGCACC38750.033.96576734
TCGCCGG36900.032.46550448
CCCGTCG36950.032.04659344
CCGTCGC36950.031.97310845
GGACGTA3400.031.73295243
GACGTAT3300.031.66100344
GCACCCC43400.031.1523936
CCCCCGA43300.031.0339519
CACCCCC44100.030.51828637
ATGGCCG12100.030.13589169
CCTATCG703.8980448E-529.5211012
AGGACCG5650.028.81628270
ACCCGCG46800.028.60902625
GGCACCC46800.028.50236735
CGATCTA1352.6921043E-1028.43705747
ACGTATC3750.027.87979745
CCCCGAC48950.027.38863820
Produced by FastQC (version 0.11.5)