FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885752
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG102640.3556785198450872No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC75850.26284309947632367No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC62310.21592292061133458No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47670.16519091037622083No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38580.1336913220540088No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36630.12693398462515143No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC29320.10160263252005024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG22250.060.948444
TCTACGG22300.060.657445
TACGGCC22450.060.252167
CTACGGC23100.058.556756
ACGGCCA25200.053.8136068
CGGCCAT25700.052.632729
GCCATAC26150.051.59537511
GCGCGGT38600.051.4499449
GGGTCTA21150.049.322982
TGGCGCG39900.049.0835315
GCGTCTA4750.047.8373762
CCATACC28150.047.80735812
CCTGGTT40600.047.6268270
GGCCATA28450.047.4242210
CATACCA28450.047.1822613
CTGAACG29450.045.76215422
CGCGGTG43700.045.5242510
GAACGCG29500.045.47936624
TACCACC29700.044.8488115
CCCGATC29800.044.56919531
Produced by FastQC (version 0.11.5)