FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968290
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG110080.3708532522091844No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59110.19913822436487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47910.16140606207614486No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35100.11824990145841545No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC33390.11248900882326188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA35550.037.88009332
TGGCCGT13150.037.2467170
GCGGCAC37650.035.6934733
CGGCACC40050.033.74123834
GTCCGTT2100.032.7992442
GCACCCC44200.031.4633236
CCCCCGA43400.031.28566719
CACCCCC45350.031.0652437
ATGGCCG12800.030.8651569
ACCCGCG45400.030.26728225
TCGCCGG36650.029.82555648
CCGTCGC37000.029.66925645
CCCGTCG36750.029.6616844
GGCACCC47550.028.72489535
CGGTTCG605.4805074E-428.6993372
CCCCGAC49300.027.54665820
GTGCCGT5000.026.9020651
AGTCCGC52850.026.1272627
CAGTCCG50900.026.0457846
AGGACCG5100.025.74216570
Produced by FastQC (version 0.11.5)