FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968290
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG103830.3497973580748512No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC77030.2595096840268303No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61720.20793116575536755No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47760.16090072061692087No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41230.1389015224253695No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36370.12252845914651196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21200.061.5417064
TCTACGG21200.061.5417065
TACGGCC21550.060.2298167
CTACGGC22850.056.7983866
ACGGCCA23900.054.3076368
CGGCCAT24850.051.95449
CGCGATA200.00742380551.6374472
GCCATAC25500.051.5751711
GCGCGGT39650.050.88299
CCATACC26050.049.56110412
CATACCA26800.048.4302513
CCGAACT6150.047.64823567
TGGCGCG42300.047.5268115
GGCCATA28150.046.59765210
GAACGCG27900.046.49860824
GGGTCTA21000.046.3917352
CCTGGTT41300.045.32708770
TGAACGC28650.045.1464423
GGTCTAC23400.045.0165023
TACCACC28700.044.85964615
Produced by FastQC (version 0.11.5)