FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938095
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG108200.36826583211230407No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58740.19992546190643937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45260.15404539335862183No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA34150.11623177603174847No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC33420.11374717291306102No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC29530.10050730149978133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA35950.037.72176432
GCGGCAC37200.036.2868833
CGGCACC38750.034.67339734
TGGCCGT13500.034.58134570
GACGTAT3800.032.07245644
GGACGTA4200.031.48565343
CCCGTCG37100.031.06718344
GCACCCC44250.030.78454836
TCGCCGG36600.030.62783248
CCGTCGC37800.029.95618245
CACCCCC45950.029.43649337
GGCACCC46250.029.21345135
CCCCCGA45750.029.06978819
CGCCCTA10350.029.04804861
ATGGCCG12500.028.5350669
TAGTCCG2800.028.2869366
ACCCGCG47350.028.0510925
CGGATAG4500.026.94545735
CGCGCTA658.617526E-426.5609828
TCGGATA4600.026.34793534
Produced by FastQC (version 0.11.5)