FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938095
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG101390.34508754822427457No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76480.26030472125646037No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61420.20904701856134675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45100.15350082281206018No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38860.13226257149615653No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36290.1235154070920103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG20200.061.1841165
GTCTACG20500.060.4566734
TACGGCC20500.060.1228567
CTACGGC21500.057.3264436
ACGGCCA23750.052.0404788
GGGTCTA19100.049.747512
CGGCCAT24650.049.7197239
GCCATAC24550.049.50155311
GCGCGGT38000.049.0127759
TGGCGCG39600.047.20472315
CCATACC25800.046.96978812
CATACCA26050.046.91389513
GAACGCG27000.045.7221124
GCGTCTA4900.045.6680952
CGCGGTG41350.045.12522510
CGTCTAC5850.044.7253073
CTGAACG27950.044.39432522
CCTGGTT39800.044.29569670
TGAACGC28050.044.2466323
ATCTCGT27500.043.95817635
Produced by FastQC (version 0.11.5)