FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761781
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG164130.4363092907322356No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129700.3447834948392796No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC108080.2873107179817219No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA101660.27024433373447315No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT58180.1546607843465635No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC52290.13900330721006884No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45130.11996976963837076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG35050.064.200394
TCTACGG35850.062.7677465
TACGGCC35750.062.455737
CTACGGC37250.060.5942766
ACGGCCA39100.057.105428
GGGTCTA31600.055.2519042
CGGCCAT40650.054.672899
GCCATAC41500.053.4712211
CCATACC42000.052.83606312
GCGCGGT60950.052.5687459
GGCCATA43300.051.56838610
CATACCA42900.051.5664713
CGGTCTA4800.051.139922
CCTGGTT63300.051.1000470
GAACGCG44800.049.3614424
CTGAACG45150.049.34757622
TGGCGCG65100.049.32640515
TACCACC45500.049.15159215
TGAACGC45250.048.94442723
GCGTCTA7600.048.6758042
Produced by FastQC (version 0.11.5)