FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782431
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG154830.4093399192212627No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC83920.22186789395497236No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG70730.18699614084169677No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG68770.18181428821834425No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC58270.15405436345038415No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT40500.1070739955335603No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39500.10443019317470696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15000.041.5531270
CGCGGCA48250.036.06890532
TCGCCGG47600.034.77948448
GCGGCAC50800.034.46903233
CCCGTCG48550.034.3325344
CCGTCGC48400.034.09260645
CGGCACC52000.033.8204134
GCACCCC57600.030.54488236
AGGACCG5550.029.88781570
TAGTCCG4300.029.7578336
CACCCCC59250.029.64474537
CCCCCGA59750.029.59127819
ATGGCCG16350.029.46759269
ACCCGCG60100.029.32282425
GGCACCC61800.028.46259135
AGTCCGC67700.026.3082777
CGTCGCC63900.025.99670446
CGCCCTA12150.025.74621661
CCCCGAC68550.025.74343120
GTCCGCC69800.025.5663158