FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782431
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG165180.4367032736353948No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC139090.36772647009291115No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC114310.30221304764052537No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA105140.27796938000984023No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT60270.15934196816809082No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC52270.1381915492972641No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG43990.11630086576595844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG30000.062.3518684
TCTACGG30100.061.6852465
TACGGCC30400.061.0765047
CTACGGC31150.059.6059656
ACGGCCA34300.054.232758
CGGCCAT34900.053.102249
GCCATAC35200.052.44835711
GCGCGGT63350.051.5773059
GGGTCTA27100.050.2776642
GGCCATA37650.049.31085610
CCATACC37800.048.9322512
CCGAACT9950.048.80815567
GCGTCTA7300.048.7935682
CCTGGTT54450.048.42260470
CATACCA38600.048.27638613
GCCGGTT10450.047.6452563
CTGAACG38800.047.44020522
TGAACGC39100.047.3510823
GAACGCG39050.047.32810224
TGGCGCG69550.046.8794315