FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3848437
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG154050.40029237843831145No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC85400.22190827081228043No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74750.1942346983983368No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG69080.1795014443526034No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC58320.15154204161325754No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT39910.10370443897093806No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39890.10365246982086493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT17300.040.7583670
CGCGGCA46600.036.83176832
GCGGCAC48800.035.17820433
TCGCCGG47250.033.9309848
CCCGTCG47250.033.78901344
CGGCACC51200.033.33601834
CCGTCGC48300.032.85433245
GCACCCC56550.030.68619236
CCCCCGA57150.030.56792319
ACCCGCG58100.029.9767125
ATGGCCG18700.029.64640469
CACCCCC58950.029.26866537
GGCACCC58500.029.24306935
AGTCCGC63700.027.7433727
CAGTCCG62150.027.6005866
GTTCCGT3650.027.5243991
AGGACCG6250.027.3989470
GTCCGTT3650.026.5272452
CCCCGAC65600.026.47533820
TCCGCCC69000.026.4143129