FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726404
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141100.37864922858605776No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC136200.3654998223488382No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC111960.3004505147590009No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG110500.29653252841076816No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA84140.22579408995911343No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT55390.14864196152644749No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC54350.14585106714140497No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT42800.1148560381536731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG24750.059.4128575
TACGGCC24700.059.4063537
GTCTACG25100.058.8567164
CTACGGC26250.056.0246246
CCGAACT8950.054.03011367
GCCATAC27900.052.4684311
ACGGCCA28450.051.8169988
GGGTCTA22250.050.0670132
GCGCGGT65950.049.1770449
CGGCCAT30150.049.009029
GCCGGTT10250.048.4869583
GGCCATA30450.048.18840810
CCATACC30900.047.48471512
CATACCA30750.047.26785313
TGGCGCG70650.046.2327615
GCGTCTA5950.045.510172
CGCGGTG74200.044.17128810
TACCACC32750.044.16418515
CGGTTCA11050.044.0478175
CGAACTT11250.044.0399868