FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705569
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG201380.5434523011175881No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145780.3934078679954415No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG93820.2531864876892051No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC70660.19068596482753392No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA70540.19036212792151488No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC52080.1405452172122554No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG51700.13951973367652848No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG50160.13536382671595104No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC48660.13131586539071327No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39030.1053279536826868No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37710.10176574771647755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA70950.037.0903832
GCGGCAC72450.036.30298233
CGGCACC73300.034.8204234
GCACCCC76850.033.38802336
TGGCCGT16000.033.3636470
ACCCGCG79600.033.24815425
CCCCCGA80050.032.95221319
CACCCCC81150.031.64316437
TCGCCGG66400.031.44943648
GGCACCC82000.031.3529835
CCCGTCG67300.031.34337844
CCGTCGC67950.030.96340445
GATAGGC23450.030.59253140
GTTCCGT3950.030.4391381
CCCCGAC87350.030.0950820
TTCCTCG11350.029.903584
AGTCCGC92350.028.9577677
TCCGCCC97100.028.4945439
TAGTCCG4700.028.4494696
CAGTCCG89550.028.4083296