FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752939
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG201020.5356335394739963No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC136680.3641945685767874No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC122240.32571805723461No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG91080.24268979591727974No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG79620.21215372805153507No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC67810.1806850577640617No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG63310.16869445519897872No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT59200.15774303818953625No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54180.1443668548835992No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG51250.1365596403245563No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA51000.13589349573760726No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49830.13277593907068566No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49710.13245618966895012No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT48130.12824615587943208No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA47890.127606657075961No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG46760.12459568354295128No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG45840.12214427146297875No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG38290.10202670493711728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT27300.056.10595770
ATGGCCG32000.038.17775369
CGCGGCA59850.037.8481432
GCGGCAC62150.036.40509833
CGGCACC62850.036.0724934
CCGTCGC60850.034.8780645
TAGTCCG3350.034.87686
TCAGGAT24200.034.86307570
TCGCCGG62450.034.36746248
CCCGTCG62300.033.98244544
GCACCCC67150.033.94793736
GTCCGTT3150.033.8292772
AGGACCG10900.033.1724770
GTTCCGT4000.032.680591
CCCCCGA70200.032.62254319
GGCACCC70350.032.3402535
CACCCCC71700.031.86456137
GTGCCGT5300.031.8043461
GTATGGC37050.030.8992667
TATGGCC37650.030.63662368
Produced by FastQC (version 0.11.5)