FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752939
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG319670.8517857604400177No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129200.3442635225352717No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126440.3369092862953541No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC110530.2945158447819163No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG89040.23725405608777547No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT65990.1758355251710726No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG62600.1668026045720434No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC59250.15787626710692607No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC52740.14052986206277265No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49580.1321097944837366No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39060.10407843026492038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG62400.063.3238375
GTCTACG62500.063.167544
TACGGCC63000.062.6116647
CTACGGC63800.061.826576
ACGGCCA65850.060.0061848
TCGCGTA307.675677E-659.20820260
GGGTCTA48550.058.9582372
CGGCCAT67800.058.2803469
GCCATAC68250.057.391111
CCTGGTT97400.057.2462370
CCATACC69850.056.02729812
GGCCATA70700.055.6938910
CATACCA70600.055.52944613
GCGTCTA12350.055.0917972
CGTCTAC14750.053.579123
TACCACC74150.052.7796515
GCGCGGT64950.052.26759
CGGTCTA7650.051.2073332
GCGGTAT1550.051.1414451
ACCACCC78400.050.0061616
Produced by FastQC (version 0.11.5)