EGAF00005319252 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005319252
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3811080
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG	20056	0.5262550248223601	No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC	14031	0.3681633552693725	No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC	12418	0.3258393946073029	No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG	9161	0.24037805556430197	No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG	8027	0.21062271062271062	No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC	6511	0.17084396024224105	No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG	6422	0.16850866421066993	No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT	5765	0.15126945642704956	No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG	5309	0.13930434417540435	No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG	5081	0.13332178804958172	No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA	5010	0.1314587990805756	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	4981	0.13069785992422095	No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT	4928	0.12930717801777974	No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA	4751	0.12466282523589114	No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT	4713	0.12366573254825404	No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG	4683	0.12287855411064581	No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG	4542	0.11917881545388709	No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG	3874	0.10165097557647701	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGGCCGT	2745	55.509315	70
CGCGGCA	6195	40.185677	32
GCGGCAC	6285	39.58195	33
AGGACCG	990	38.98434	70
CGGCACC	6435	38.52062	34
ATGGCCG	3200	38.510113	69
TCGCCGG	6390	36.886402	48
CCGTCGC	6265	36.730183	45
CCCGTCG	6320	36.661057	44
GGCACCC	6990	35.67646	35
GCACCCC	7030	35.63757	36
GACGTAT	480	35.51425	44
CGGATAG	505	34.930317	35
GTTCCGT	435	34.799408	1
CACCCCC	7250	34.343876	37
GGACGTA	500	34.07056	43
CCCCCGA	7350	33.99862	19
CGTATCA	525	33.18627	46
TCGGATA	540	32.65119	34
TATGGCC	3570	32.075417	68

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content