FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786421
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG199520.5269355943250895No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG84910.2242487034590184No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC71820.18967779863887296No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC62980.16633121356552796No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA61140.16147174336926612No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG60240.15909482859935545No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC59810.15795919154262034No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54670.1443843671900193No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT45940.12132829392188561No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA39700.10484835151717149No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39270.1037127144604364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT16000.041.28132270
CGCGGCA58300.040.0906132
CGTACTA1150.039.121652
GCGGCAC60000.039.07910533
CGGCACC62200.037.53904734
TCGCCGG58550.037.20958748
CCCGTCG59100.036.91880844
CCGTCGC59950.036.5299145
GTTCCGT4150.035.1426241
GCACCCC67250.034.74039536
GTGCCGT5600.034.724261
CCCCCGA68900.034.3916919
ACCCGCG69650.033.9547925
CACCCCC69650.033.35235237
GGCACCC71800.032.67352735
ATGGCCG16950.031.18192769
CCCCGAC76850.030.79502320
TAGTCCG3850.030.5415656
AGTCCGC83900.028.4832277
CAGTCCG81500.027.9216676