FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784538
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG202130.534094254041048No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG86690.22906362678879166No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC73510.1942377114458885No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC63960.16900345563976368No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC62280.16456434048224644No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA60410.1596231825390576No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG59480.1571658152197177No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG55250.1459887574123975No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT45280.11964472281689337No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39710.10492694220536297No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA39280.10379074011147464No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG38360.10135979609664376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT20350.042.13876370
GTTCCGT3700.036.5968931
CGCGGCA59550.035.87536232
GCGGCAC61050.035.00318533
ATGGCCG19750.034.52762669
CGGCACC64200.033.40444634
TCGCCGG60950.033.2870548
CCGTCGC61400.032.89314745
CCCGTCG61550.032.8656244
CCCCCGA71450.030.50422919
GCACCCC71000.030.36385536
ACCCGCG71200.030.24822825
CACCCCC71650.030.19732937
CGTACGT1950.030.16032819
GACGTAT2550.030.03836344
GGCACCC74200.029.09574335
TACGGGT10800.028.5003456
AGGACCG5800.028.36636770
TATGGCC23000.028.3039268
GTATGGC22600.028.05867467