FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784538
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG154520.40829290127355045No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC139810.3694242203407655No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC116740.30846565683843047No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA111730.29522758127940585No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT70230.18557086756692626No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56530.1493709403895535No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45760.12091304143332687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG31500.062.2429285
GTCTACG31450.062.2319344
TACGGCC32250.060.6881947
CTACGGC32750.059.7585036
ACGGCCA35100.056.0560768
CGGCCAT35750.054.7466939
GCCATAC35900.054.0363411
GCGCGGT67400.052.8952069
CCATACC37600.051.68517312
CGTCTAC8400.051.4574553
CCGAACT11350.050.87811767
CATACCA38300.050.83082213
GGCCATA38950.050.16011410
GGGTCTA27600.049.8645062
CGGTTCA11650.049.568765
GAACGCG39450.049.13483424
GCGTCTA7750.049.080452
CCTGGTT54750.049.02563570
TGAACGC40050.048.3916923
TGGCGCG74050.048.04905715