FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539309
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG148290.41898008905128087No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG69230.19560315304484577No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC55310.15627344207584024No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA54000.15257215462114215No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC51190.14463275176030124No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50430.1424854399545222No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA43420.12267931395648134No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36670.10360779462883855No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC36640.1035230323207157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14600.038.52638670
CGCGGCA44100.035.2045932
GCGGCAC45250.034.39719833
GTTCCGT3950.033.3844221
TCGCCGG43400.033.15672748
AGGACCG5850.032.62277270
CCCGTCG44000.032.5627644
CGGCACC47600.032.55936434
CCGTCGC44900.031.9241345
ATGGCCG14350.030.95603869
GCACCCC52250.029.94287536
CCCCCGA52100.029.86927219
CACCCCC52900.029.32013937
ACCCGCG53600.028.98894925
GGCACCC57500.027.02183735
TACGGGT9250.026.5338756
CGCCCTA18850.026.50663461
GTACGGG9400.026.4782145
CCCCGAC59150.026.37064720
TCGCCGT2900.026.3258251