FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529060
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG147800.4188084079046545No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG70450.19962822961355148No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC56120.15902251591075245No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA53800.15244852736989453No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG51280.14530781567896267No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC50970.14442939479634803No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA43190.1223838642584711No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC38080.10790408777408148No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36570.1036253279910231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14800.042.42984470
CGCGGCA44150.037.0474932
GCGGCAC45650.036.06865733
AGGACCG6550.036.0478870
TCGCCGG43650.035.14327248
CCCGTCG44350.034.75183544
CCGTCGC44750.034.45251545
CGGCACC47550.034.2702434
ATGGCCG16050.030.8303669
CCCCCGA53800.030.77883119
GCACCCC53400.030.66627736
ACCCGCG54700.030.1105825
CACCCCC54800.029.8258937
GTGCCGT5200.029.349341
GGCACCC56800.028.94660235
CGCCCTA18700.028.2215261
GTTCCGT4750.027.7484681
CCCCGAC59900.027.5329120
CGCTTCC8450.027.502181
CGTACTA1306.204573E-926.5832272