FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533014
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG135560.3836950547040006No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC98200.2779496486569258No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC78130.22114262779598381No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA68290.19329105404054442No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56290.1593257201924476No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44950.12722847970599607No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT37680.10665114828302406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG27650.062.2394875
TACGGCC27900.061.8047757
GTCTACG28400.060.7106364
CTACGGC29300.058.7345356
ACGGCCA32000.054.3179868
CGGCCAT32150.054.0637939
GCCATAC32050.053.58556711
CCATACC33400.051.62514512
GGGTCTA25950.051.278492
CCTGGTT47100.051.21767870
CATACCA34500.050.27960213
GCGCGGT46850.050.155539
GGCCATA34550.050.10824210
GAACGCG34350.049.9465424
TGAACGC35050.049.23532523
CCCGATC35050.049.1250731
CTGAACG35600.048.75918222
GCGTCTA6700.048.4914172
ATCTCGT35200.048.3753635
GCCCGAT35700.048.2196730