FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555930
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG64090.25075021616397947No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC40780.1595505354215491No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG35350.1383058221469289No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG31260.12230381896217815No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA30870.12077795557781316No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC30270.11843047344802088No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC26650.10426733126494075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT11200.042.4901170
AGGACCG4900.034.8366170
CGCGGCA19700.033.59483732
GCGGCAC20350.032.3610433
CCCGTCG18800.031.62240244
CGGCACC20800.031.16284634
CCGTCGC19100.030.95161445
TCGCCGG18950.030.87641748
ATGGCCG12350.029.46741969
GTTCCGT1900.029.1962811
GATAGGC13700.027.62258740
CCCCCGA24500.026.67256219
ACCCGCG24750.026.56104325
TCGCCGT1850.026.23721
GCACCCC25550.026.05842836
CAGTCCG25050.025.6648186
AGTCCGC25800.025.5886047
AACGGCG4950.025.3378549
CACCCCC26800.024.85326637
CGGAACG5050.024.80430246