FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549178
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG70550.27675587973848825No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC40320.15816863318293192No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG36550.14337955215367465No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG32120.12600140123600626No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA31960.1253737479297248No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC31270.12266699304638592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG4300.043.11253470
TGGCCGT11250.038.4499970
CGCGGCA23450.035.3167832
CCCGTCG22400.034.61971344
GCGGCAC24150.034.01215733
TCGCCGG22750.033.99397748
CGGCACC24150.033.73575634
CCGTCGC23300.033.1463145
AGCGAAC2600.030.55980568
CGTACGT1052.2815584E-829.63975119
CCCCCGA28650.028.60520719
CGCCCTA12600.028.44939261
CGACGGA500.006737046428.39340667
GCACCCC29400.028.19818136
GACGTAT2600.028.0874744
ACCCGCG29450.027.96643825
GGCACCC29950.027.21226135
CACCCCC30800.026.92652137
GTGCCGT3400.026.4677311
AGTCCGC31850.026.2689117
Produced by FastQC (version 0.11.5)