FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549178
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG122070.4788602443611235No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC75600.2965661872179973No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC65630.2574555405703329No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA56770.2226992387349961No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34820.13659305077950618No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG31380.12309850469445446No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT27700.10866247864998049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21400.061.7070924
TCTACGG21450.061.563255
TACGGCC21550.061.4379927
CTACGGC22700.058.3254936
ACGGCCA23950.055.7143758
CGGCCAT24950.053.2042279
GCCATAC25100.053.01567511
GGGTCTA19150.052.000982
CCATACC25950.051.1459412
CCTGGTT37550.049.65379770
CATACCA26800.049.52377313
GGCCATA27100.049.3581610
GCGCGGT37150.049.317679
GAACGCG27150.048.03474424
GCGTCTA5200.047.87592
CTGAACG27450.047.7458722
CCCGATC27250.047.6663831
TACCACC27850.047.41306715
CCGAACT4500.047.346667
ATCTCGT27650.047.0313935