FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619325
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG70640.2696878012465044No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC41680.1591249654013916No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG38940.14866425510389128No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG34260.13079705649356227No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC31560.12048905729529553No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA31520.12033634619606196No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC26930.10281274755900853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAC21250.037.355933
CGCGGCA21550.037.152632
TGGCCGT13100.036.65669670
TATCGGA1051.6370905E-1136.2227678
TCGCCGG21700.035.47308748
CCCGTCG21850.035.3373144
CGGCACC23050.034.75082434
GTCCGAT501.8366148E-434.58092
CCGTCGC22500.034.48096545
GTTATCG1056.2937033E-1032.9297876
CGCCCTA14150.030.7916661
ACCCGCG27300.029.92726125
CCCCCGA28000.029.15343719
GTTCCGT2300.028.6149271
GCACCCC28300.028.43808436
CACCCCC29000.028.237737
AGTCCGC29250.028.015657
ATGGCCG12800.027.69108669
GGCACCC29450.027.44223435
CAGTCCG28650.027.0334576