FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619325
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG122720.46851765244862703No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC77600.29625953251314746No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC65910.2516297137621334No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA57430.21925496072461417No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC35810.13671461158886353No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG29390.11220448016187377No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT28680.1094938581504777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23550.062.9672624
TCTACGG24150.061.5459865
TACGGCC24050.061.382447
CTACGGC25000.059.1802866
ACGGCCA26350.056.1557858
CGGCCAT27200.054.01969
GCCATAC27450.053.0238311
CCTGGTT40950.052.18304470
GCGCGGT37550.051.6516579
GGGTCTA22900.051.4725382
CCATACC28300.051.30908212
CGGTCTA3150.050.4782142
GAACGCG29550.049.64740824
TGAACGC29700.049.27639823
CTGAACG29700.049.15614322
CATACCA30050.048.7812313
CGCGGTG40300.048.12704510
CCCGATC30450.048.0133131
CCGAACT5250.047.98623367
GGCCATA30850.047.85243610