FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594121
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG69940.2696096288492325No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC40680.15681612384310525No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG38090.1468320097636155No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG33470.12902250897317433No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA31840.12273907038260744No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC30790.11869145656659809No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC26970.10396585201692596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT12150.044.79487270
GCGGCAC21700.034.66521533
CGGCACC21550.034.59147334
CGCGGCA21500.034.49730732
CCCGTCG21000.033.94157444
GTTCCGT3400.033.6267931
CCGTCGC21750.032.78198645
TATCGGA958.607458E-932.760288
TCGCCGG21750.032.5088348
AGGACCG4300.032.2399270
ATGGCCG13350.031.83551269
CGTTCGT802.6740563E-630.3150621
CCCCCGA26400.028.56375919
ACCCGCG26700.028.26299325
GCACCCC26150.028.25503236
GTAGCGT1503.45608E-1127.66423610
GGCACCC27950.027.2967635
TAGCGTC1555.0931703E-1126.77184111
GTCCGTT2650.026.0993442
TTATCGG1208.336974E-825.9352237
Produced by FastQC (version 0.11.5)