FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260235
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG156490.47999607390264815No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC85230.2614228728910646No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC81680.25053408726671544No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62020.19023168575271412No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49730.15253501664757294No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG47730.14640048953526358No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41960.128702378816251No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT37780.11588121715152436No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35900.11011476166595353No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT35450.10873449306568392No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT34280.10514579470498292No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG32720.10036086355738161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT12250.042.43480770
CGCGGCA49700.035.3911632
GCGGCAC50700.034.64569533
CGGCACC52250.033.35940634
CCCGTCG49900.033.0048544
TCGCCGG50050.032.99926848
CCGTCGC49600.032.9482545
TAGTCCG2850.032.6049356
AGGACCG4850.032.57452470
CCCCCGA55650.031.50872619
GTTATCG1650.031.2875656
ACCCGCG56650.031.0764125
GCACCCC56700.030.88928236
CGCCCTA8750.030.70329961
CCCCGAC58450.030.01086620
CACCCCC59700.029.23580437
ATGGCCG14200.029.0599869
GGCACCC60150.028.87442635
GTACGGG10250.028.5403635
GATAGGC28600.028.42956440