FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265626
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG110360.3379443941222908No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC97810.29951378388094657No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA86990.26638077967287127No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC82070.25131475557825667No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50230.15381430696595383No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT49900.15280378095960773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG23200.060.555665
GTCTACG23300.060.4435424
TACGGCC23500.059.929137
CTACGGC24450.057.5997166
ACGGCCA26300.053.8115238
GCCATAC26950.052.38508211
CGGCCAT27400.051.6512079
CCGAACT8850.050.80831567
GCGCGGT48550.050.782379
CATACCA29000.048.68200313
TGGCGCG51200.048.42077615
CCATACC29150.048.3133712
GGCCATA29750.047.8026810
CTGAACG29650.046.99941322
CGCGGTG53050.046.6045410
GGGTCTA21450.046.5535962
GCCGGTT9400.045.7886733
TACCACC31200.045.13754715
CCTGGTT43150.045.06934470
CGAACTT10650.045.0389368
Produced by FastQC (version 0.11.5)