FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244503
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG155420.47902560114754095No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC86020.26512535201847554No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC82100.25304337829245344No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61840.1905992998003084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50010.15413762909142018No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG48190.14852814129005276No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42380.13062093023184135No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT36380.11212811330425645No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT36360.11206647058116452No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA36040.11108018701169331No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT34600.10664191094907292No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA33980.1047309865332225No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG32520.10023106774751019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14650.038.30053370
CGCGGCA46000.035.1514132
GCGGCAC47200.034.11895433
CGGCACC48750.032.97538834
CCCGTCG46050.032.5214744
TATCGGA1500.032.148138
CCGTCGC46550.032.11487245
TCGCCGG46900.032.09447548
ATGGCCG14500.031.63518569
GTTCCGT3200.031.4599761
GTACGGC5850.031.20605714
CCCCCGA52500.031.1885519
ACCCGCG52650.030.82756425
TACGCGT450.004650335330.6309742
GCACCCC53450.030.3031636
TACGGCC6200.029.9999815
CCCCGAC56300.029.2757420
CGCGAGA6400.028.59799825
CACCCCC56550.028.59264637
CGCCCTA9650.028.1822761