FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244503
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG109040.33607612629730965No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC95740.29508371544116313No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA82120.2531050210155454No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC79290.24438257569803448No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49960.15398352228369028No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47770.1472336441051218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23800.061.3506244
TCTACGG24000.060.8450055
TACGGCC24700.059.3986057
CTACGGC25200.058.0842936
ACGGCCA26200.056.5237278
CGGCCAT27950.052.861459
GCGCGGT50850.052.7606479
GCCATAC28450.051.8105711
GGGTCTA22050.050.6167142
TGGCGCG53500.050.2698115
CCATACC29850.049.3767912
CATACCA30300.048.3025113
CCGAACT7100.048.24532367
CGCGGTG56150.048.08650210
GCGTCTA5300.046.7965852
GGCCATA32050.045.9909810
TACCACC32050.045.77112215
CCTGGTT45500.045.04587670
CTGAACG32400.044.9188222
GAACGCG32450.044.6619124
Produced by FastQC (version 0.11.5)