FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474554
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG186110.5356370918397009No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC105360.3032331631628117No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC85420.24584450263256807No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG73080.21032915303661995No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG72430.20845840933829204No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA57480.16543130427675035No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT48730.1402482160300286No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47390.13639160594424493No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA45440.13077937484926122No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT41590.11969881602070366No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40110.11543927652297244No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG36460.10493433114005424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA54500.038.1564532
GCGGCAC56400.036.81532733
CCCGTCG54050.036.24656344
TCGCCGG54750.035.7630348
CCGTCGC54750.035.66685545
CGGCACC58400.035.4991734
CCCCCGA61600.034.1981619
TAGTCCG2850.034.0290036
ACCCGCG63200.033.3544825
GCACCCC62800.033.1369136
CACCCCC63700.032.72893537
GGCACCC67200.031.01355735
CCCCGAC69100.030.5386920
TGGCCGT12350.030.28285470
TTCCTCG9250.029.5947934
CCGAACG1700.028.65716644
CGCTTCC9750.028.5229021
GTCGGTT2450.028.2874534
GATAGGC30250.028.27684840
GCATTAG31000.027.59759912