FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474554
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC144870.4169455993488661No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA124750.3590388867175471No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC120430.346605636291737No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG97240.27986325726985395No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69000.1985866387455771No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC39380.11333828744638881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG20250.058.6614574
TACGGCC20200.058.4637577
TCTACGG20600.057.8328975
CTACGGC21700.054.741686
GCGCGGT66150.051.7785849
GCCGGTT11300.051.483823
CCGAACT10950.051.2612567
ACGGCCA23550.050.8825538
GGGTCTA19000.050.120922
GCCATAC23900.049.2580511
TGGCGCG69500.049.17745615
CGGTTCA12000.049.0626955
CCATACC24400.048.2542212
CGGCCAT24500.048.2027749
CGCGGTG71700.047.91552410
CATACCA24750.047.29200413
CGAACTT12600.046.70003568
CGGCAAT2700.045.1076851
CCGGTTC13300.044.2670944
GCGTCTA4100.043.9197042
Produced by FastQC (version 0.11.5)